Nanotopography and oral bacterial adhesion on titanium surfaces: in vitro and in vivo studies.

The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey's post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.

Nanotopography and oral bacterial adhesion on titanium surfaces: in vitro and in vivo studies

Abstract The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey's post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.

Otimização de resultados através da colocação do Implante Dentário e associação de osso liofilizado e membrana reabsorvível û relato de caso / Optimizing results through dental implant installation associated with lyophilized bone and resorbable membrane û case report

A realização de enxertia óssea convencional visando corrigir defeitos ósseos, para posterior colocação de implantes dentários é uma opção viável e previsível, mas que aumenta o tempo e a morbidade dos tratamentos implantodônticos. Sendo assim, a realização da implantação mesmo em áreas com deficiente volume ósseo, associada à colocação de biomateriais e membranas reabsovíveis é uma opção para reduzir a duração e a morbidade do tratamento. De acordo com a literatura, esse método quando bem indicado e executado, proporciona resultados semelhantes à enxertia convencional. Diante do exposto, o objetivo do presente trabalho foi relatar e discutir o caso clínico de um paciente que apresentava perda óssea em região anterior de mandíbula e que foi tratado através de implantação, colocação de biomaterial e membrana reabsorvível sobre a superfície exposta do implante, no mesmo momento cirúrgico. Os resultados pós-operatórios demonstram estabilidade, tecidos moles mantidos em posição e volume gengival adequado, o que vem a evidenciar o sucesso do plano de tratamento executado.

The performance of conventional bone grafting aiming to correct bone defects, for later placement of dental implants is a viable and predictable option, but it increases implantodontic treatments time and morbidity. Thus, performing the implantation even in areas with poor bone volume associated with the placement of biomaterials and resorbable membranes is an option for reducing treatment duration and morbidity. According to the literature this method when well indicated and performed provides similar results to conventional grafting. Considering all this, the objective of this study was to report and discuss the case of a patient with bone loss in the anterior jaw, who was treated through implantation, placement of biomaterial and resorbable membrane on the exposed surface of the implant at the same surgical time. The postoperative results have demonstrated stability, soft tissue held in place and adequate gingival volume, which shows the success of the treatment plan implemented.